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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Topic 1: Laboratory Operations- Quality and safety in the clinical lab
  • 1. Laboratory safety and regulations
    • 2. Quality control and assurance
      Topic 2: Blood Banking / Immunohematology- Blood group systems and antibody identification
      • 1. ABO and Rh systems
        • 2. Crossmatching procedures
          Topic 3: Clinical Chemistry- Metabolic and analytical testing
          • 1. Enzymes and electrolytes
            • 2. Endocrine and metabolic panels
              Topic 4: Microbiology- Clinical microbiological techniques
              • 1. Bacteriology and organism identification
                • 2. Culture and sensitivity testing
                  Topic 5: Urinalysis and Body Fluids- Fluid analysis and interpretation
                  • 1. CSF and other body fluids
                    • 2. Urine chemical and microscopic examination
                      Topic 6: Hematology- Blood cell analysis and disorders
                      • 1. Anemias and leukemias overview
                        • 2. CBC and differential interpretation

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          1. What is the first thing a phlebotomist should do in the event of an accidental needle stick?

                          A) decontaminate the site and fill out an incident report
                          B) leave the area so that the patient does not notice the injury
                          C) go to the employee health service and get a tetanus booster
                          D) check the patient's medical records


                          2. A blood culture to evaluate septicemia is performed in:

                          A) microbiology.
                          B) toxicology.
                          C) hematology.
                          D) urinalysis.


                          3. Hemolytic anemia, myelodysplasia, and liver disease may each fit this peripheral blood picture. Each of these conditions can display peripheral blood macrocytosis. It is easy to observe the overall larger size of the red blood cells in this image compared to the normal lymphocyte also present.
                          When macrocytes are present, they should be examined for their shape (round vs. oval), the hemoglobin content (central pallor), and whether or not there are any inclusions present in the cell.
                          Iron deficiency would not be the correct answer in this case, since this condition is associated with microcytosis instead.
                          The complete blood count was obtained from a patient recently admitted to the emergency room. The red blood cell indices obtained revealed an MCV of 115 femtoliters (fL) (normal range 80 - 90 fL). The patient met the criteria for a peripheral blood smear examination. A representative field is shown on the right.

                          Which of the following conditions may be indicated by the results seen on this peripheral blood smear?

                          A) Liver disease
                          B) Hemolytic anemia
                          C) Myelodysplasia
                          D) Iron deficiency


                          4. The Western Blot Assay is used as a confirmatory test for which of the following:

                          A) Anti-HIV-1
                          B) CMV Antibody
                          C) Anti-HCV
                          D) HBsAg


                          5. Isolation and detection of Gardnerella vaginalis from vaginal secretions is improved by which of the following?

                          A) cold enrichment
                          B) utilization of human blood agar
                          C) incubation at 35-37ºC
                          D) utilization of Thayer-Martin agar


                          Solutions:

                          Question # 1
                          Answer: A
                          Question # 2
                          Answer: A
                          Question # 3
                          Answer: A,B,C
                          Question # 4
                          Answer: C
                          Question # 5
                          Answer: B

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